PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Id2 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.5~9(S-P-V-R-S) derived from Human Id2.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 15kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extract from mouse heart tissue using Id2 Antibody #AB21620
Background
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
The MGC Project TeamGenome Res. 14:2121-2127(2004)