PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total EGFR protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.676~680 (K-R-T-L-R) derived from Human EGFR.
Target NameEGFR
Alternative Names Receptor tyrosine-protein kinase ErbB-1; ERBB1;
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from HUVEC and MDA cells using EGFR(Ab-678) Antibody #AB21193.
Background
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia
Doherty JK, et al. (1999) Proc Natl Acad Sci U S A; 96(19): 10869-10874
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
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