GRB2 Monoclonal Antibody

Description

Product Name GRB2 Monoclonal Antibody
Host Species Mouse
Clonality Monoclonal
Clone No. 3B2-G2-E5
Isotype IgG1
Applications WB
Species Reactivity Hu Rt Ms Mk
Specificity This antibody detects endogenous levels of GRB2 and does not cross-react with related proteins.
Immunogen Type Recombinant Protein
Immunogen Description Purified recombinant human GRB2 protein fragments expressed in E.coli.
Target Name GRB2
Alternative Names Abundant SRC homology; Adapter protein GRB2; ASH; Ash protein; EGFRBP GRB2; Epidermal growth factor receptor binding protein; Epidermal growth factor receptor binding protein GRB2; GRB 2; GRB2 adapter protein; Grb2; GRB2_HUMAN; Grb3 3;
Accession No. Uniprot: P62993
Gene ID: 2885
SDS-PAGE MW 25kd
Formulation Ascites
Storage store at -20˚C

Application Details

Western blotting: 1:500

Images

Western blot detection of GRB2 in Jurkat,Raji,PC-12&3T3 cell lysates and using GRB2 antibody(1:500 diluted).Predicted band size: 25KDa Observed band size: 25KDa
Western blot detection of GRB2 in MCF7,3T3,COS7,C6 and Hela cell lysates and using GRB2 antibody(1:300 diluted).Predicted band size: 25KDa.Observed band size: 25KDa.

Background

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

Published Papers

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