PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms
SpecificityEPHA1 (Phospho-Tyr599) Antibody detects endogenous levels of EPHA1 only when phosphorylated at Tyr599
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human EPHA1 (Phospho-Tyr599)