p73(Phospho-Tyr99) Antibody

Description

Product Name p73(Phospho-Tyr99) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of p73 only when phosphorylated at tyrosine99.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 99 (S-P-Y(p)-A-Q) derived from Human P73.
Target Name p73
Modification Phospho-Tyr99
Alternative Names TP73; TRP73; p53-related protein; p73-alpha;
Accession No. Swiss-Prot: O15350
NCBI Protein: NP_001119712.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 80kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from 293 cells untreated or treated with UV using p73(Phospho-Tyr99) Antibody #AB11058.

Background

This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined.

Yuan ZM, et al.(1999) Nature.399(6738): 814-817.

Published Papers

let us know

Customer Reviews and Rankings

No comment