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GST Tag AntibodyAB35540

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Description

Product Name GST Tag Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antigen affinity purification.
Applications WB
Specificity The antibody detects transfected proteins contanining GST tag.
Immunogen Type Recombinant Protein
Immunogen Description Full length fusion protein
Target Name GST Tag
SDS-PAGE MW 63kd
Concentration 2.5mg/ml
Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:1000-1:8000

Images

Gel: 10%SDS-PAGE
Lysate: 2ug Recombinant GST tag protein
Primary antibody: 1/1000 dilution
Secondary antibody dilution: 1/8000
Exposure time: 15 seconds

Background

GST (Glutathione S-Transferase) is a 26kDa protein which was widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

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