TUBB3 Antibody

Description

Product Name TUBB3 Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antigen affinity purification.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous levels of total TUBB3 protein.
Immunogen Type Recombinant Protein
Immunogen Description Fusion protein corresponding to a region derived from internal residues of human Tubulin beta-3 chain
Target Name TUBB3
Alternative Names TUBB4
Accession No. Swiss-Prot#: Q13509
NCBI Gene ID: 10381
Gene Accssion: BC000748
SDS-PAGE MW 50kd
Concentration 0.5mg/ml
Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:25-1:50

Images

Gel: 8%SDS-PAGE
Lysate:30ug Human brain glioma tissue
Primary antibody: 1/500 dilution
Secondary antibody dilution: 1/8000
Exposure time: 10 seconds
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using #AB35539 at dilution 1/25.
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using #AB35539 at dilution 1/25.

Background

his gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6

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