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FLAG tag AntibodyAB35535

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Description

Product Name FLAG tag Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antigen affinity purification.
Applications WB
Specificity The antibody detects transfected proteins contanining FLAG tag.
Immunogen Type Peptide
Immunogen Description Synthetic peptide: DYKDDDDK conjugated to KLH
Target Name FLAG tag
Alternative Names DDDDK epitope tag; DYKDDDDK epitope tag
SDS-PAGE MW 54kd
Concentration 1.4mg/ml
Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:1000-1:5000

Images

Gel: 8%SDS-PAGE
Lysate:40ug 293T cells transfected with DYKDDDDK-tagged protein vector
Primary antibody: 1/500 dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds

Background

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

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