HA Tag Antibody

Description

Product Name HA Tag Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antigen affinity purification.
Applications WB
Specificity The antibody detects transfected proteins contanining HA tag.
Immunogen Type Peptide
Immunogen Description Synthetic peptide: YPYDVPDYA (Human influenza hemagglutinin-HA-epitope) conjugated to KLH.
Target Name HA Tag
Alternative Names HA epitope tag; Hemagglutinin Tag
SDS-PAGE MW 55kd
Concentration 0.5mg/ml
Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:500-1:5000

Images

Gel: 8%SDS-PAGE
Lysate: 2ug Recombinant HA tagged protein
Primary antibody: 1/500 dilution
Secondary antibody dilution: 1/8000
Exposure time: 1 second

Background

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

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