PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of STAT4 only when phosphorylated at tyrosine 693.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 693 (K-G-Y(p)-V-P) derived from Human STAT4.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 85kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Images
Western blot analysis of extracts from HepG2 cells untreated or treated with IL-4 using STAT4(Phospho-Tyr693) Antibody #AB11047.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT4(Phospho-Tyr693) Antibody #AB11047(left) or the same antibody preincubated with blocking peptide(right).
Background
Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
Strausberg R L, et al. (2002) Proc Natl Acad Sci U S A. 99(26):16899-16903.