PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total alpha-Tubulin protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.437-441(V-D-S-V-E) derived from Human alpha-Tubulin.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 52kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from Hela and HT29 cells using alpha-Tubulin Antibody #AB21581.
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the a-chain.