STAT1(Phospho-Tyr701) Antibody

Description

Product Name STAT1(Phospho-Tyr701) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms
Specificity The antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 701 (T-G-Y(p)-I-K) derived from Human STAT1.
Target Name STAT1
Modification Phospho-Tyr701
Alternative Names CANDF7, ISGF-3, STAT91
Accession No. Swiss-Prot: P42224
NCBI Protein: NP _009330.1
SDS-PAGE MW 84,91kd
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 84,91kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1(Phospho-Tyr701) Antibody #AB11044(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from MEF cells untreated or treated with interferon-ɤ (IFNɤ) using STAT1 (Phospho-Tyr701) Antibody #AB11044.
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using STAT1 (Phospho-Tyr701) Antibody #AB11044.

Background

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

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