PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total WISP2 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.242~246(G-R-S-P-Q) derived from Human WISP2.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 27kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from A549, A431 cells using WISP2 Antibody #AB21668.
Background
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.