PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous levels of total Blimp-1 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.124~128(D-T-V-P-K) derived from Human Blimp-1
Target NameBlimp-1
Alternative NamesPRDM1; PRDI-BF1; MGC118922; MGC118923; MGC118924
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 95 100kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from JK and Raji cells using Blimp-1 Antibody #AB21643.
Background
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells. "NANOS3 function in human germ cell development."
Julaton V.T., Reijo Pera R.A. Hum. Mol. Genet. 20:1-13(2011)