PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous levels of total Synaptophysin protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.305~309(A-P-T-S-F) derived from Human Synaptophysin
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 38kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from Rat and Mouse brain tissue and C6 cells using Synaptophysin Antibody #AB21651.
Background
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity "Characterization of a multidomain adaptor protein, p140Cap, as part of a pre-synaptic complex."
Ito H., Atsuzawa K., Sudo K., Di Stefano P. J. Neurochem. 107:61-72(2008) "A systematic, large-scale resequencing screen of X-chromosome coding exons in mental retardation."
Tarpey P.S., Smith R., Pleasance E., et al. Nat. Genet. 41:535-543(2009)