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PPARg AntibodyAB21649

  Catalog No. Package Size Price
AB21649-1 50ul $189.00
AB21649-2 100ul $279.00
AB21649-4 25ul $119.00

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Description
  • Product Name PPARg Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous levels of total PPARg protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.50~54 (L-S-V-M-E) derived from Human PPARg
  • Target Name PPARg
  • Alternative Names GLM1; NR1C3; PPARG1; PPARG2; PPARG
  • Accession No. Swiss-Prot: P37231
    NCBI Protein: NP_005028.4
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 53 57kd
Western blotting: 1:500~1:1000

Images
  • PPARg Antibody - Absci

    Western blot analysis of extracts from Hela, 293, 3T3 and MCF-7 cells using PPARg Antibody #AB21649.

Background
Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. "Identification, characterization, and tissue distribution of human peroxisome proliferator-activated receptor (PPAR) isoforms PPARgamma2 versus PPARgamma1 and activation with retinoid X receptor agonists and antagonists."

Mukherjee R., Jow L., Croston G.E., et al. J. Biol. Chem. 272:8071-8076(1997) "Cloning and characterization of RAP250, a nuclear receptor coactivator."
Caira F., Antonson P., Pelto-Huikko M., et al. J. Biol. Chem. 275:5308-5317(2000) "Identification of protein arginine methyltransferase 2 as a coactivator for estrogen receptor a."
Qi C., Chang J., Zhu Y.,et al. J. Biol. Chem. 277:28624-28630(2002)
    Please let us know if you have published research using #AB21649 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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