PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total CCR5(CD195) protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.11~15(D-I-N-Y-Y) derived from Human CCR5(CD195).
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 41kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from Mouse liver tissue using CCR5(CD195) Antibody #AB21666.
Background
Receptor for a number of inflammatory CC-chemokines including MIP-1-a, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
Samson M., Labbe O., Mollereau C. Biochemistry 35:3362-3367(1996)
Raport C.J., Gosling J., Schweichart V.L. J. Biol. Chem. 271:17161-17166(1996) Combadiere C., Ahuja S.K., Tiffany H.L., Murphy P.M.J. Leukoc. Biol. 60:147-152(1996)