GATA1(Phospho-Ser142) Antibody

Description

Product Name GATA1(Phospho-Ser142) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC IF
Species Reactivity Human Mouse Rat
Specificity The antibody detects endogenous level of GATA1 only when phosphorylated at serine 142.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 142 (R-L-S(p)-P-D) derived from Human GATA1.
Target Name GATA1
Modification Phospho-Ser142
Alternative Names GAT1; GATA1; GF-1; NF-E1;
Accession No. Swiss-Prot: P15976
NCBI Protein: NP_002040.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from HL60 cells untreated or treated with PMA using GATA1(Phospho-Ser142) Antibody #AB11041.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1(Phospho-Ser142) Antibody #AB11041(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using GATA1(Phospho-Ser142) Antibody #AB11041.

Background

GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.

Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672.
Trainor C D, et al. (1990) Nature. 343: 92-96.
Nichols K E, et al. (2000) Nat Genet. 24: 266-270.

Published Papers

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