PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Rt
SpecificityThe antibody detects endogenous level of total a-Internexin protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.491~495 (E-T-T-I-S
Target Namea-Internexin
Alternative NamesNEF5; NF-66; TXBP-1; FLJ18662; MGC12702
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 66kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extract from Rat brain and Rat hippocampus Tissue using a-Internexin Antibody #AB21638
Background
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. Science 316:1160-1166(2007)