PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Periostin protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa. 125~129( R-E-E-I-E) derived from Mouse Periostin.
Target NamePeriostin
Alternative NamesPOSTN; OSF2; OSF-2; PN; osteoblast specific factor
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 93kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extract from Rat brain and Mouse brain Tissue using Periostin Antibody #AB21451
Background
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
Horiuchi K., Amizuka N., Takeshita S., Takamatsu H., Katsuura M., Kudo A.J. Bone Miner. Res. 14:1239-1249(1999)
Sugiura T., Takamatsu H., Kudo A., Amann E. Protein Expr. Purif. 6:305-311(1995)