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TIF1b AntibodyAB21635

  Catalog No. Package Size Price
AB21635-1 50ul $189.00
AB21635-2 100ul $279.00
AB21635-4 25ul $119.00

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Description
  • Product Name TIF1b Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB
  • Species Reactivity Hu Mk
  • Specificity The antibody detects endogenous level of total TIF1b protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.827~831 (L-S-G-G-P) derived from Human TIF1b.
  • Target Name TIF1b
  • Alternative Names KAP1, RNF96
  • Accession No. Swiss-Prot: Q13263
    NCBI Protein: NP_005753.1
  • SDS-PAGE MW 110kd
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 110kd
Western blotting: 1:500~1:1000

Images
  • TIF1b Antibody - Absci

    Western blot analysis of extract from JK, T98G, COS7 and Hela cells using TIF1b Antibody #AB21635

Background

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger.

Friedman J.R., Fredericks W.J., Jensen D.E. Genes Dev. 10:2067-2078(1996)
Moosmann P.R., Georgiev O., le Douarin B., Bourquin J.-P. Nucleic Acids Res. 24:4859-4867(1996)
Agata Y., Matsuda E., Shimizu A.J. Biol. Chem. 274:16412-16422(1999)

    Please let us know if you have published research using #AB21635 so that we can cite your reference.
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    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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