CD22 Antibody

Description

Product Name CD22 Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of total CD22 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.839~843 (N-V-D-Y-V) derived from Human CD22.
Target Name CD22
Alternative Names SIGLEC2; FLJ22814; SIGLEC-2; MGC130020;
Accession No. Swiss-Prot: P20273
NCBI Protein: NP_001762.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 140kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extract from Raji cells using CD22 Antibody #AB21492

Background

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to a-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Doody G.M., Justement L.B., Delibrias C.C., Matthews R.J., Lin J., Thomas M.L., Fearon D.T. Science 269:242-244(1995)
Tuscano J.M., Engel P., Tedder T.F., Agarwal A., Kehrl J.H.Eur. J. Immunol. 26:1246-1252(1996)
Law C.L., Sidorenko S.P., Chandran K.A., Zhao Z., Shen S.H., Fischer E.H., Clark E.A.J. Exp. Med. 183:547-560(1996)

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