PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Fatty Acid Synthase (FASN) protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.2503~2507 (E-P-R-V-S) derived from Human Fatty Acid Synthase (FASN).
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 273kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extract from Rat brain tissue, Hela, Raji, P19 cells using Fatty Acid Synthase(FASN) Antibody #AB21605
Background
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
Jayakumar A., Tai M.-H., Huang W.-Y. Natl. Acad. Sci. U.S.A. 92:8695-8699(1995)
Chi A., Valencia J.C., Hu Z.-Z., Watabe H. Proteome Res. 5:3135-3144(2006)