Welcome     Login   Register   Message Board   
Your Position: Home > Neuroscience Research > GAPDH Antibody

GAPDH AntibodyAB21612

  Catalog No. Package Size Price
AB21612-1 50ul $189.00
AB21612-2 100ul $279.00
AB21612-4 25ul $119.00

Quantity:  Total:   
 

Review:comment rank 5 pdf

Availability: promotionYes

add promotion
Description
  • Product Name GAPDH Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total GAPDH protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.252~256(P-A-K-Y-D) derived from Human GAPDH.
  • Target Name GAPDH
  • Alternative Names G3PD; GAPD; MGC88685
  • Accession No. Swiss-Prot: P04406
    NCBI Protein: NP_002037.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 37kd
Western blotting: 1:5000~1:10000

Images
  • GAPDH Antibody - Absci

    Western blot analysis of extract from Hela, 293 and 3T3 cells using GAPDH Antibody #AB21612

Background

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Ercolani L., Florence B., Denaro M., Alexander M. J. Biol. Chem. 263:15335-15341(1988)
Tisdale E.J.J. Biol. Chem. 277:3334-3341(2002)

    Please let us know if you have published research using #AB21612 so that we can cite your reference.
Customer Reviews and Rankings
  • No comment
  • Total 0 results, divided into1 pages. First Prev Next Last

    My Review

    Username: Anonymous user
    E-mail:
    Ratings:
    Content:
    Verification code: captcha
     
    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


    © 2017, AbSci All Rights Reserved. E-mail: info@abscitech.com