PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total ERG protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.84~88(G-S-R-N-S) derived from Human ERG.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 52 55kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extract from P19, JK, Hela and 293 cells using ERG Antibody #AB21480
Background
Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.