PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total CD11c protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.1155~1159(Q-T-P-S-P)derived from Human CD11c.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 145kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extract from JK, HL-60 cells using CD11c Antibody #AB21491
Background
Integrin a-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.J. Proteome Res. 8:651-661(2009)