CD44 Antibody

Description

Product Name CD44 Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IF
Species Reactivity Hu
Specificity The antibody detects endogenous level of total CD44 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.734~738(Q-N-V-D-M) derived from Human CD44.
Target Name CD44
Alternative Names LHR; MDU2; MDU3; MIC4;
Accession No. Swiss-Prot: P16070
NCBI Protein: NP_000601.3
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 80kd
Western blotting: 1:500~1:1000

Immunofluorescence:1:100~1:200

Images

Western blot analysis of extract from Hela cells using CD44 Antibody #AB21471
Immunofluorescence staining of methanol-fixed NCI-h446 cells showing cytoplasmic staining using CD44 Antibody #AB21471.

Background

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

Teriete P.,et.al(2004) Mol. Cell 13:483-496 Bartolazzi A. (2003)Melanoma Res. 13:325-337
Liu T.et.al (2005)J. Proteome Res. 4:2070-2080

Published Papers

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