ATF2(Phospho-Ser112 or 94) Antibody

Description

Product Name ATF2(Phospho-Ser112 or 94) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of ATF-2 only when phosphorylated at serine 112 or 94.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 112 or 94 (D-L-S(p)-P-L) derived from Human ATF2.
Target Name ATF2
Modification Phospho-Ser112 or 94
Alternative Names CREB2; CREBP1;
Accession No. Swiss-Prot: P15336
NCBI Protein: NP_001871.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 65-75 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from Huvec cells untreated or treated with Anisomycin using ATF2(Phospho-Ser112 or 94) Antibody #AB11033.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Ser112 or 94) Antibody #AB11033(left) or the same antibody preincubated with blocking peptide(right).

Background

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.
Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089.
Gupta S, et al. (1995) Science. 267: 389-393.
van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.
Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.

Published Papers

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