Welcome     Login   Register   Message Board   
Your Position: Home > Neuroscience Research > Tyrosine Hydroxylase(Ab-31) Antibody

Tyrosine Hydroxylase(Ab-31) AntibodyAB21294

  Catalog No. Package Size Price
AB21294-1 50ul $189.00
AB21294-2 100ul $279.00
AB21294-4 25ul $119.00

Quantity:  Total:   
 

Review:comment rank 5 pdf

Availability: promotionYes

add promotion
Description
  • Product Name Tyrosine Hydroxylase(Ab-31) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB
  • Species Reactivity Ms Rt
  • Specificity The antibody detects endogenous levels of total Tyrosine Hydroxylase protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.29~33(V-T-S-P-R)derived from Mouse Tyrosine Hydroxylase.
  • Target Name Tyrosine Hydroxylase
  • Alternative Names TYH; DYT14; DYT5b; TH;
  • Accession No. Swiss-Prot: P07101
    NCBI Protein: NP_954986.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 55-60kd
Western blotting: 1:500~1:1000

Images
  • Tyrosine Hydroxylase(Ab-31) Antibody - Absci

    Western blot analysis of extract from rat brain and mouse brain using Tyrosine Hydroxylase(Ab-31) Antibody #AB21294.

Background
Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms . This enzyme is required for embryonic development since TH knockout mice die before or at birth . Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH . Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels.

Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62.
Bodeau-P
    Please let us know if you have published research using #AB21294 so that we can cite your reference.
Customer Reviews and Rankings
  • No comment
  • Total 0 results, divided into1 pages. First Prev Next Last

    My Review

    Username: Anonymous user
    E-mail:
    Ratings:
    Content:
    Verification code: captcha
     
    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


    © 2017, AbSci All Rights Reserved. E-mail: info@abscitech.com