TFE3 Antibody

Description

Product Name TFE3 Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB
Species Reactivity Human Mouse Rat
Specificity The antibody detects endogenous levels of total TFE3 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.303~307 (L-D-V-Y-S) derived from Human TFE3.
Target Name TFE3
Alternative Names BHLHE33; TFEA; RCCP2
Accession No. Swiss-Prot: P19532
NCBI Protein: NP_006512.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 70kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from 293 cells using TFE3 Antibody #AB21431.

Background

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.

Clark J., Lu Y.-J., Sidhar S.K., Parker C., Gill S. Oncogene 15:2233-2239(1997) Weterman M.A.J., Wilbrink M., Proc. Natl. Acad. Sci. U.S.A. 93:15294-15298(1996)

Published Papers

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