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ATF2(Phospho-Thr71 or 53) AntibodyAB11031

  Catalog No. Package Size Price
AB11031-1 50ul $189.00
AB11031-2 100ul $279.00
AB11031-4 25ul $119.00

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Description
  • Product Name ATF2(Phospho-Thr71 or 53) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of ATF-2 only when phosphorylated at threonine 71 or 53.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of threonine 71 or 53 (T-P-T(p)-P-T) derived from Human ATF2.
  • Target Name ATF2
  • Modification Phospho-Thr71 or 53
  • Alternative Names CREB2; CREBP1;
  • Accession No. Swiss-Prot: P15336
    NCBI Protein: NP_001871.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 65-75 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • ATF2(Phospho-Thr71 or 53) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Thr71 or 53) Antibody #AB11031(left) or the same antibody preincubated with blocking peptide(right).

  • ATF2(Phospho-Thr71 or 53) Antibody - Absci

    Western blot analysis of extracts from HUVEC cells untreated or treated with Anisomycin using ATF2 (Phospho-Thr71 or 53) Antibody #AB11031.

Background

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

Sevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465.
Waetzig G H, et al. (2002) J Immunol. 168(10): 5342-5351.
Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089.
Gupta S, et al. (1995) Science. 267: 389-393.
Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.

    Please let us know if you have published research using #AB11031 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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