Lin28 Antibody

Description

Product Name Lin28 Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous levels of total Lin28 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa. 1~5 (M-G-S-V-S) derived from Human Lin28
Target Name Lin28
Alternative Names CSDD1; LIN-28; LIN28A; ZCCHC1;
Accession No. Swiss-Prot: Q9H9Z2
NCBI Protein: NP_078950.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 26kd
Western blotting: 1:1000

Images

Western blot analysis of extracts from HepG2 cells using Lin28 Antibody #AB21426.

Background

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells.

Heo I., Joo C., Mol. et al., Cell 32:276-284(2008)
Heo I., Joo C., Kim Y.-K., et al., Cell 138:696-708(2009)
Viswanathan S.R., Powers J.T., et al., Nat. Genet. 41:843-848(2009)

Published Papers

let us know

Customer Reviews and Rankings

No comment