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PKCb(Phospho-Thr641) AntibodyAB11172

  Catalog No. Package Size Price
AB11172-1 50ul $189.00
AB11172-2 100ul $279.00
AB11172-4 25ul $119.00

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Description
  • Product Name PKCb(Phospho-Thr641) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous levels of PKCb only when phosphorylated at threonine 641.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of threonine 641 (E-L-T(p)-P-T) derived from Human PKCb
  • Target Name PKCb
  • Modification Phospho-Thr641
  • Alternative Names PKCB; PRKCB1; PRKCB2
  • Accession No. Swiss-Prot: P05771
    NCBI Protein: NP_002729.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 82kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • PKCb(Phospho-Thr641) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCb(phospho-Thr641) antibody(#AB11172).

  • PKCb(Phospho-Thr641) Antibody - Absci

    Immunofluorescence staining of methanol-fixed MCF7 cells using PKCb(phospho-Thr641) antibody(#AB11172, Red).

  • PKCb(Phospho-Thr641) Antibody - Absci

    Western blot analysis of extracts from JK cells untreated or treated with PMA using PKCβ (phospho-Thr641) antibody #AB11172.

Background

Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.

Zhang Y, et al. (2006) Mol Cell Biol ; 26: 6748-6761
Castoria G, et al. (2004) Mol Cell Biol ; 24: 7643-7653
Marcil J, et al. (1999) Biochem J ; 337:185-192
Bornancin F, et al. (1996) Curr Biol ; 6:1114-1123.

    Please let us know if you have published research using #AB11172 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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