JunD(Phospho-Ser255) Antibody

Description

Product Name JunD(Phospho-Ser255) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of JunD only when phosphorylated at serine 255.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 255 (G-E-S(p)-P-P) derived from Human JunD.
Target Name JunD
Modification Phospho-Ser255
Alternative Names AP-1
Accession No. Swiss-Prot: P17535
NCBI Protein: NP_005345.3
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 38kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from 293 cells using JunD(Phospho-Ser255) Antibody #AB11028(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD(Phospho-Ser255) Antibody #AB11028(left) or the same antibody preincubated with blocking peptide(right).

Background

Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.

Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.

Published Papers

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