OCT-4 Antibody

Description

Product Name OCT-4 Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of total OCT-4 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.232~236 (R-K-R-T-S) derived from OCT-4
Target Name OCT-4
Alternative Names Otc3; OTF3; OTF4; OTF-3;
Accession No. Swiss-Prot: Q01860
NCBI Protein: NP_002692.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 45kd
Western blotting: 1:1000

Images

Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell using OCT-4 Antibody #AB21424.

Background

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency

Pesce, M. and Scholer, H.R. (2001) Stem Cells 19, 271-278.
Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.

Published Papers

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