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Myc-Tag Mouse Monoclonal AntibodyABT504

  Catalog No. Package Size Price
ABT504-1 50ul $189.00
ABT504-2 100ul $279.00

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Description
  • Product Name Myc-Tag Mouse Monoclonal Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 9E10
  • Isotype IgG1
  • Purification Antibodies were produced from mice ascites by injecting mice with a monoclonal cell line which was fusioned by mouse spleen and SP2/0 myeloma cell. Spleen cells were isolated from mice by immunizing with synthetic peptide and KLH conjugates.
  • Applications WB
  • Specificity The mouse mAb detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected cells.
  • Immunogen Type Peptide
  • Immunogen Description Peptide sequence around aa. 410-419 (EQKLISEEDL) derived from human c-Myc.
  • Target Name Myc-Tag
  • Accession No. NCBI Protein: NP_002458.2
  • Concentration 1.0mg/ml
  • Formulation Supplied in mice ascites
  • Storage Store at 4°C for short term use. Store at -20°C for long term preservation. Avoid freezing and thawing repeatly.
Application Details

Western blotting: 1:5000~1:10000
 

Images
  • Myc-Tag Mouse Monoclonal Antibody - Absci

    Western blot analysis of extracts from 293 cells transfected with Myc-CDK2 using Myc-Tag mouse mAb #ABT504.

Background

Epitope tags are artificial epitopes useful for the labeling and detection of proteins. Epitope tags - short amino acid sequences are 'fused' to the N- or C-terminus of the protein.

Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.

    Please let us know if you have published research using #ABT504 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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