c-kit(Ab-936) Antibody

Description

Product Name c-kit(Ab-936) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous level of total c-Kit protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.934~938 (H-I-Y-S-N) derived from Human c-Kit.
Target Name c-kit
Alternative Names KIT; CD117; SCFR; PBT;
Accession No. Swiss-Prot: P10721
NCBI Protein: NP_000213.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 145kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from HL60 cells using c-kit(Ab-936) Antibody #AB21539.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit(Ab-936) Antibody #AB21539(left) or the same antibody preincubated with blocking peptide(right).

Background

This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

Martin, F.H. et al. (1990) Cell 63, 203-11.
Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
Nocka, K. et al. (1990) EMBO J 9, 1805-13.

Published Papers

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