PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsIHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total CDC2 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.17~21 (V-V-Y-K-G) derived from Human CDC2.
Target NameCDC2
Alternative NamesCDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2(Ab-19) Antibody #AB21529(left) or the same antibody preincubated with blocking peptide(right).
Background
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
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McGowan, C.H. and Russell, P. (1993) EMBO J. 12, 75-85.