PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total PLC-g1 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa. 769~773 (P-D-Y-G-A) derived from Human PLC-g1.
Target NamePLCg1
Alternative NamesPhosphoinositide phospholipase C; Phospholipase C-gamma-1;
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Images
Western blot analysis of extracts from 3T3 cells using PLC-g1(Ab-771) Antibody #AB21523.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PLC-g1(Ab-771) Antibody #AB21523(left) or the same antibody preincubated with blocking peptide(right).
Background
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.