PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Her3/ErbB3 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa. 1326~1330 (P-D-Y-W-H) derived from Human Her3/ErbB3.
Target NameHer3/ErbB3
Alternative Names Tyrosine kinase-type cell surface receptor HER3
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Images
Western blot analysis of extracts from A431 cells using Her3/ErbB3(Ab-1328) Antibody #AB21510.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Her3/ErbB3(Ab-1328) Antibody #AB21510(left) or the same antibody preincubated with blocking peptide(right).