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H2B AntibodyAB21417

  Catalog No. Package Size Price
AB21417-1 50ul $189.00
AB21417-2 100ul $279.00
AB21417-4 25ul $119.00

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Description
  • Product Name H2B Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total H2B protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.41~45 (Y-V-Y-K-V) derived from Human H2B.
  • Target Name H2B
  • Alternative Names H2BQ; GL105; H2B.1; H2BFQ; H2BGL105
  • Accession No. Swiss-Prot: P33778
    NCBI Protein: NP_003519.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 14kd
Western blotting: 1:500~1:1000

Images
  • H2B Antibody - Absci

    Western blot analysis of extracts from Hela and 293 cells using H2B antibody #AB21417.

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Borchert GM,et al. (2010) PLoS One.5(7):e11641.
Pennini ME,et al.(2010) PLoS Pathog.6(7):e1000995.
Lu C,et al. (2010) Sci China Life Sci.53(6):663-8.

    Please let us know if you have published research using #AB21417 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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