Smad3(Ab-425) Antibody

Description

Product Name Smad3(Ab-425) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total Smad3 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.423~427 (C-S-S-V-S) derived from Human Smad3.
Target Name Smad3
Alternative Names JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3
Accession No. Swiss-Prot: P84022
NCBI Protein: NP_001138574.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 52kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blotting：1:500~1:1000

Images

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3(Ab-425) Antibody #AB21325(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Smad3(Ab-425) Antibody #AB21325.
Western blot analysis of extracts from HT29 and Hela cells using Smad3 Antibody #21325.

Background

Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Shi W, et al. J Cell Sci. 2007 Apr 1;120(Pt 7):1216-24
Seong HA, et al. J Biol Chem. 2007 Apr 20;282(16):12272-89
Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1191-200
LeClair RJ, et al. Circ Res. 2007 Mar 30;100(6):826-33

Published Papers

let us know

Customer Reviews and Rankings

No comment