PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Smad2 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa. 463~467 (C-S-S-M-S) derived from Human Smad2.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Images
Western blot analysis of extracts from Hela cells using Smad2(Ab-467) Antibody #AB21322.
Immunofluorescence staining of methanol-fixed Hela cells using Smad2(Ab-467) Antibody #AB21322.
Background
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420