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c-Jun(Phospho-Thr91) AntibodyAB11021

  Catalog No. Package Size Price
AB11021-1 50ul $189.00
AB11021-2 100ul $279.00
AB11021-4 25ul $119.00

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Description
  • Product Name c-Jun(Phospho-Thr91) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of c-Jun only when phosphorylated at threonine 91.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of threonine 91 (T-T-T(p)-P-T) derived from Human c-Jun.
  • Target Name c-Jun
  • Modification Phospho-Thr91
  • Alternative Names AH119; AP1; Jun A; c-Jun; p39
  • Accession No. Swiss-Prot: P05412
    NCBI Protein: NP_002219.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • c-Jun(Phospho-Thr91) Antibody - Absci

    Western blot analysis of extracts from 293 cells untreated or treated with UV using c-Jun(Phospho-Thr91) Antibody #AB11021.

  • c-Jun(Phospho-Thr91) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Thr91) Antibody #AB11021(left) or the same antibody preincubated with blocking peptide(right).

Background
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Binetruy B, et al. (1991) Nature. 351: 122-127.
Smeal T, et al. (1991) Nature. 354:494-496.
Derijard B, et al. (1994) Cell. 76:1025-1037.
Kyriakis J M, et al. (1994) Nature. 369: 156-160.
    Please let us know if you have published research using #AB11021 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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