MAPKAPK-2(Ab-334) Antibody

Description

Product Name MAPKAPK-2(Ab-334) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total MAPKAPK-2 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.332~336 (P-Q-T-P-L) derived from Human MAPKAPK-2.
Target Name MAPKAPK-2
Alternative Names MAP kinase-activated protein kinase 2
Accession No. Swiss-Prot: P49137
NCBI Protein: NP_004750.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 49kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from 293 cells using MAPKAPK-2(Ab-334) Antibody #AB21308 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed Hela cells using MAPKAPK-2(Ab-334) Antibody #AB21308.
Western blot analysis of extracts from JK, 3T3 and C6 cells using MAPKAPK-2 (Ab-334) Antibody #AB21308

Background

MAPKAPK-2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Rouse, J. et al. (1994) Cell 78, 1027-1037.
Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

Published Papers

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