PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total IKK-bprotein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.197~201 (Q-K-Y-T-V) derived from Human IKK-b.
Target NameIKK-b
Alternative NamesI-kappa-B kinase 2; I-kappa-B-kinase beta; IKK2; IkBKB; NFKBIKB
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 87kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Images
Western blot analysis of extracts from MDA cells using IKK-b(Ab-199) Antibody #AB21305.
Immunofluorescence staining of methanol-fixed Hela cells using IKK-b(Ab-199) Antibody #AB21305.
Background
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
Chen RA, et al. PLoS Pathog. 2008 Feb 8;4(2):e22
Yen CJ,et al. Cancer Res. 2008 Apr 15;68(8):2632-40