Rel(Phospho-Ser503) Antibody

Description

Product Name Rel(Phospho-Ser503) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications IF
Species Reactivity Hu
Specificity The antibody detects endogenous level of Rel only when phosphorylated at serine 503.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 503 (T-S-S(p)-D-S) derived from Human Rel.
Target Name Rel
Modification Phospho-Ser503
Alternative Names C-Rel
Accession No. Swiss-Prot: Q04864
NCBI Protein: NP_002899.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 78kd
Immunofluorescence: 1:100~1:200

Images

Immunofluorescence staining of methanol-fixed Hela cells using Rel(Phospho-Ser503) Antibody #AB11020.

Background

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 (PubMed 18037997)).

Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
Haskill, S. et al. (1991) Cell 65, 1281-9.
Thompson, J.E. et al. (1995) Cell 80, 573-82.

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