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Synaptotagmin 1 (Ab-309) AntibodyAB21292

  Catalog No. Package Size Price
AB21292-1 50ul $189.00
AB21292-2 100ul $279.00
AB21292-4 25ul $119.00

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Description
  • Product Name Synaptotagmin 1 (Ab-309) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total Synaptotagmin 1 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.307~311 (G-L-S-D-P) derived from Human Synaptotagmin 1.
  • Target Name Synaptotagmin 1
  • Alternative Names SYT1; SytI; Synaptotagmin I; P65; SYT
  • Accession No. Swiss-Prot: P21579
    NCBI Protein: NP_001129277.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 60-65kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images
  • Synaptotagmin 1 (Ab-309) Antibody - Absci

    Immunofluorescence staining of methanol-fixed Hela cells using Synaptotagmin 1 (Ab-309) Antibody #AB21292.

  • Synaptotagmin 1 (Ab-309) Antibody - Absci

    Western blot analysis of extracts from mouse brain and rat brain tissue using Synaptotagmin 1 (Ab-309) Antibody #AB21292.

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse

Gustavsson N, et al. Proc Natl Acad Sci U S A. 2008 Mar 11; 105(10):3992-7.
Cnops L, et al. Cereb Cortex. 2008 May; 18(5):1221-31.
Lynch KL, et al. Mol Biol Cell. 2007 Dec; 18(12):4957-68.

    Please let us know if you have published research using #AB21292 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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