a-Synuclein(Ab-136) Antibody

Description

Product Name a-Synuclein(Ab-136) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total a-Synuclein protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa. 134~138 (Q-D-Y-E-P) derived from Human α-Synuclein.
Target Name a-Synuclein
Alternative Names NACP; SYN; SYUA; alpha-synuclein;
Accession No. Swiss-Prot: P37840
NCBI Protein: NP_000336.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from Hela and 3T3 cells using a-Synuclein(Ab-136) Antibody #AB21278.
Immunofluorescence staining of methanol-fixed Hela cells using a-Synuclein(Ab-136) Antibody #AB21278.

Background

SncA is a member of the synuclein family of structurally related proteins that are prominently expressed in the central nervous system, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and SncA and Snc-Beta inhibit phospholipase D2 selectively. SncA may serve to integrate presynaptic signaling and membrane trafficking. Aggregated SncA proteins form brain lesions that are hallmarks of neurodegenerative synucleinopathies. Defects in SncA play a role in the pathogenesis of Parkinson disease. SncA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer disease. SncA shares 95% sequence homology with rat SncA. Rat SncA is specifically expressed in brain and is associated with synaptosomal membranes in neurons

Takahashi T, et al. J Biol Chem 2003 Oct 24; 278(43): 42225-33
Ahn BH, et al. J Biol Chem 2002 Apr 05; 277(14): 12334-42
Negro A, et al. FASEB J 2002 Feb; 16(2): 210-2
Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119.

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