GAP43(Ab-41) Antibody

Description

Product Name GAP43(Ab-41) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total GAP43 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.38~43 (Q-A-S-F-R) derived from Human GAP43.
Target Name GAP43
Alternative Names B-50; BASP2; NEUM; PP46; axonal membrane protein GAP-43
Accession No. Swiss-Prot: P17677
NCBI Protein: NP_001123536.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from mouse brain tissue using GAP43(Ab-41) Antibody #AB21273 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GAP43(Ab-41) Antibody #AB21273(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using GAP43(Ab-41) Antibody #AB21273.

Background

GAP43 encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Rachael L. Neve, et,al. (1998) J. Neurosci; 18: 7757.
Yiping Shen, et,al. (2002) J. Neurosci; 22: 239.
Chantal Gamby, et,al. (1996) J. Biol. Chem; 271: 26698.

Published Papers

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